17.2kD Subunit / Anti-NADH dehydrogenase subunit 17.2 kD, mitochondrial antibody

  Description:

Rabbit polyclonal antibody

  Background:

Complex I is the largest protein complex of the oxidative phosphorylation system in mitochondrial and it catalyzes NADH-quinone oxidoreduction. Complex I represents the main entrance site for electrons into the respiratory electron transfer chain. In Arabidopsis, Complex I have at least 49 subunits and 17.2 kD subunit (AT3G03100) may be one of the subunit.

  Synonyms:

17.2kD Subunit, B17.2

  Immunogen:

KLH-conjugated synthetic peptide of 17.2kD Subunit derived from Arabidopsis thaliana AT3G03100.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

18 / 16 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Brassica rapa, and 80-99% homologues with the sequence in Populus trichocarpa, Medicago truncatula, Glycine max.
For more species homologues information, please contact tech support at tech@phytoab.com.

10 μg mitochondria protein from Arabidopsis thaliana.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.