CAK2At / Anti-Cyclin-dependent kinase D-3 antibody

  Description:

Rabbit polyclonal antibody

  Background:

Plants possess two types of CAKs, CDKD and CDKF, both of which exhibit CDK-activating kinase activities in vitro and in yeast cells. CDKD is closely related to vertebrate-type CAKs in terms of amino acid sequence similarity and enzyme activity; In Arabidopsis, there is three CDKDs:CDKD;1 ( AT1G73690), CDKD;2 (AT1G66750), CDKD;3 (AT1G18040).

  Synonyms:

CAK2At, AT;CDCKD;3, CAK2AT, CDKD1;3, CYCLIN-DEPENDENT KINASE D1;3

  Immunogen:

KLH-conjugated synthetic peptide of CAK2At protein derived from Arabidopsis thaliana AT1G18040.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Applications: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

 45 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity:  

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Brassica napus.
The sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in CDKD1;1 (AT1G73690).
For more species homologues information, please contact tech support at tech@phytoab.com.

Lane 1: 20 μg total protein from Arabidopsis thaliana leaf.
Lane 2: 40 μg total protein from Arabidopsis thaliana leaf.
Lane 3: 80 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.