FNR1 / Anti-Ferredoxin--NADP reductase, leaf isozyme 1, chloroplastic, C-terminal antibody

  Description:

Rabbit polyclonal antibody

  Background:

Two distinct ferredoxin-NADP(+)-oxidoreductase (FNR) isoforms were found in chloroplasts of Arabidopsis thaliana, FNR-1 (AT5G66190) and FNR-2 (AT1G20020). The FNR proteins are present in both chloroplast stroma and thylakoid membranes in chloroplasts but are more abundant in the stroma.

  Synonyms:

FNR1, ATLFNR1, FERREDOXIN-NADP(+)-OXIDOREDUCTASE 1, LEAF FNR 1, LEAF-TYPE CHLOROPLAST-TARGETED FNR 1, LFNR1

  Immunogen:

KLH-conjugated synthetic peptide derived from C-terminal of FNR1 protein in Arabidopsis thaliana AT5G66190.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum 
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Applications: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

40 / 34 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Brassica napus, Vitis vinifera. 
For more species homologues information, please contact tech support at tech@phytoab.com.

Lane 1: 12 μg stromal protein from Arabidopsis thaliana leaf. 
Lane 2: Thylakoid membrane protein from Arabidopsis thaliana leaf containing 5 μg of chlorophyll. 
Lane 3: 10 μl total chloroplast protein from Arabidopsis thaliana leaf. 
Lane 4: 18 μg total protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.