GAPDH / Anti-Glyceraldehyde-3-phosphate dehydrogenase GAPC, cytosolic antibody

  Description:

Rabbit polyclonal antibody

  Background:

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Plants contain both cytosolic and chloroplastic GAPDHs (glyceraldehyde-3-phosphate dehydrogenases). In Arabidopsis thaliana, cytosolic GAPDH is involved in the glycolytic pathway and is represented by two differentially expressed isoforms (GAPC1 AT3G04120 and GAPC2 AT1G13440) that are 98% identical in amino acid sequence.

  Synonyms:

GAPDH, GAPC, GAPC, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C

  Immunogen:

KLH-conjugated synthetic peptide of GAPDH derived from Arabidopsis thaliana AT3G04120 and AT1G13440.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150 µl of sterile water.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:4000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

37 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Brassica rapa, Medicago truncatula, Oryza sativa, Vitis vinifera, Glycine max, Spinacia oleracea, Zea mays, Triticum aestivum, Gossypium raimondii, Physcomitrium patens, Populus trichocarpa, Nicotiana tabacum, Solanum lycopersicum, Solanum tuberosum, Setaria viridis, Sorghum bicolor, Hordeum vulgare, Leymus chinensis, Cucumis sativus.
For more species homologues information, please contact tech support at tech@phytoab.com.

Lane 1: 15 μg cytosolic protein from Arabidopsis thaliana.
Lane 2: 30 μg cytosolic protein from Arabidopsis thaliana.
Lane 3: 15 μg total protein from Arabidopsis thaliana.
Lane 4: 30 μg total protein from Arabidopsis thaliana.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:4000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.