IAA14 / Anti-INDOLE-3-ACETIC ACID INDUCIBLE 14 antibody

  Description:

Rabbit polyclonal antibody

  Background:

IAA14 is a member of the Aux/IAA protein family. It is involved in lateral root development. This protein is localized to the nucleus. It is expressed in stele and root tip epidermis. It functions as a negative regulator of ARF7/19.

  Synonyms:

IAA14, INDOLE-3-ACETIC ACID INDUCIBLE 14, SLR, SOLITARY ROOT, Auxin-responsive protein IAA14

  Immunogen:

KLH-conjugated synthetic peptide of IAA14 derived from Arabidopsis thaliana AT4G14550.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl 0.01M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

25 / 34 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.

Lane 1: 3.75 μg nuclear protein from Arabidopsis thaliana.
Lane 2: 7.5 μg nuclear protein from Arabidopsis thaliana.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.