PBA1 / Anti-20S proteasome β subunit 1 antibody

  Description:

Rabbit polyclonal antibody

  Background:

The plant 20S proteasome β subunit 1 (PBA1) was shown to have caspase-3-like activity using biotin-DEVD-fmk pull-down and activity assay in downregulated lines.

  Synonyms:

PBA1

  Immunogen:

KLH-conjugated synthetic peptide derived from N-terminal section of PBA1 protein in Arabidopsis thaliana AT4G31300.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150 µl of sterile water.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:5000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

25 / 26 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity:  

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Nicotiana tabacum, Solanum lycopersicum, Solanum tuberosum, Brassica rapa, Cucumis sativus, Brassica napus, Medicago truncatula, Physcomitrium patens, and 80-99% homologues with the sequence in Spinacia oleracea, Vitis vinifera, Gossypium raimondii, Populus trichocarpa.
For more species homologues information, please contact tech support at tech@phytoab.com.

15 μl cytosolic protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.