PsbO / Anti-PsbO subunit of oxygen evolving complex of Photosystem II antibody

  Description:

Rabbit polyclonal antibody

  Background:

PsbO is a subunit of the oxygen evolving complex of Photosystem II, encoded by the PsbO (sll0427) . In Arabidopsis thaliana, the PsbO proteins are encoded by two genes: PsbO1 (AT5G66570) and PsbO2 (AT3G50820). PsbO1 is the major isoform in Arabidopsis.

  Synonyms:

PsbO

  Immunogen:

Recombinant protein of PsbO (1-274aa) derived from Synechocystis sp. PCC 6803 (sll0427) .

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

30.4 / 35 kDa

  Confirmed Reactivity: 

Synechocystis sp. PCC 6803

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.

Lane 1-2 is thylakoid membrane protein from Synechocystis sp. PCC 6803 cells containing 2.5 μg and 5 μg of protein, respectively.
Electrophoresis: 12% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.