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- GAPDH / Anti-Glyceraldehyde-3-phosphate dehydrogenase GAPC2, cytosolic antibody
Product Information
Description: |
Rabbit polyclonal antibody |
Background: |
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Plants contain both cytosolic and chloroplastic GAPDHs (glyceraldehyde-3-phosphate dehydrogenases). In Arabidopsis thaliana, cytosolic GAPDH is involved in the glycolytic pathway and is represented by two differentially expressed isoforms (GapC1 AT3G04120 and GapC2 AT1G13440) that are 98% identical in amino acid sequence. |
Synonyms: |
GAPDH, GAPC-2, GAPC2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2 |
Immunogen: |
KLH-conjugated synthetic peptide of GapC2 (AT1G13440) derived from Arabidopsis thaliana. |
Form: |
Lyophilized |
Quantity: |
150 µg |
Purification: |
Immunogen affinity purified |
Reconstitution: |
Reconstitution with 150µl of 0.01M sterile PBS. |
Stability & |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: |
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: |
Western Blot (1:1000-1:2000) |
Expected/apparent MW: |
37 kDa |
Confirmed Reactivity: |
Arabidopsis thaliana |
Predicted Reactivity: |
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica rapa subsp. Chinensis, Solanum tuberosum, Brassica napus, Solanum lycopersicum, Solanum tuberosum, and 80-99% homologues with the sequence in Triticum aestivum, Glycine max, Hordeum vulgare subsp. Vulgare, Zea mays, Nicotiana tabacum, Populus trichocarpa, Gossypium raimondii, Cucumis sativus. |
Application Example
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Lane 1: 5 μg total protein from Arabidopsis thaliana leaf.
|
Write Your Own Review
Type | No |
---|---|
SKU | PHY0303A |
Size | 150 μg |
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