GAPDH / Anti-Glyceraldehyde-3-phosphate dehydrogenase GAPC2, cytosolic antibody

  Description:

Rabbit polyclonal antibody

  Background:

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Plants contain both cytosolic and chloroplastic GAPDHs (glyceraldehyde-3-phosphate dehydrogenases). In Arabidopsis thaliana, cytosolic GAPDH is involved in the glycolytic pathway and is represented by two differentially expressed isoforms (GapC1 AT3G04120 and GapC2 AT1G13440) that are 98% identical in amino acid sequence.

  Synonyms:

GAPDH, GAPC-2, GAPC2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2

  Immunogen:

KLH-conjugated synthetic peptide of GapC2 (AT1G13440) derived from Arabidopsis thaliana.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

37 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica rapa subsp. Chinensis, Solanum tuberosum, Brassica napus, Solanum lycopersicum, Solanum tuberosum, and 80-99% homologues with the sequence in Triticum aestivum, Glycine max, Hordeum vulgare subsp. Vulgare, Zea mays, Nicotiana tabacum, Populus trichocarpa, Gossypium raimondii, Cucumis sativus.
For more species homologues information, please contact tech support at tech@phytoab.com.

Lane 1: 5 μg total protein from Arabidopsis thaliana leaf.
Lane 2: 10 μg total protein from Arabidopsis thaliana leaf.
Lane 3: 25 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.