PsbA / Anti-D1 subunit of Photosystem II, N-terminal antibody

  Description:

Rabbit polyclonal antibody

  Background:

The D1 protein of Photosystem II (PSII), encoded by the PsbA genes, is an indispensable component of oxygenic photosynthesis. Due to strongly oxidative chemistry of PSII water splitting, the D1 protein is prone to constant photodamage requiring its replacement, whereas most of the other PSII subunits remain ordinarily undamaged.

  Synonyms:

PsbA, 32 kDa thylakoid membrane protein, photosystem II protein D1

  Immunogen:

KLH-conjugated synthetic peptide derived from N-terminal of D1 subunit in Arabidopsis thaliana ATCG00020.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

39 / 31 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana, Glycine max, Zea mays, Oryza sativa, Hordeum vulgare

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Nicotiana tabacum, Populus trichocarpa, Solanum tuberosum, Cucumis sativus, and 80-99% homologues with the sequence in Triticum aestivum, Vitis vinifera, Gossypium raimondii, Medicago truncatula, Sorghum bicolor, Physcomitrella patens, Chlamydomonas reinhardtii.
For more species homologues information, please contact tech support at tech@phytoab.com.

Lane 1: thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.1μg chlorophyll.
Lane 2: 50μg total protein from Arabidopsis thaliana leaf.
Lane 3: 10μg total protein from Glycine max leaf.
Lane 4: 50μg total protein from Glycine max leaf.
Lane 5: 10μg total protein from Zea mays leaf.
Lane 6: 50μg total protein from Zea mays leaf.
Lane 7: 50μg total protein from Oryza sativa leaf.
Lane 8: 50μg total protein from Hordeum vulgare leaf.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.