PsaN / Anti-PsaN subunit of photosystem I antibody

  Description:

Rabbit polyclonal antibody

  Background:

Photosystem I (PSI) is a multisubunit complex that catalyses the final stage of the light reactions in chloroplasts. PsaN is the only subunit of photosystem I located entirely in the thylakoid lumen. It may be involved in the interaction between plastocyanin and the photosystem I complex.

  Synonyms:

PsaN, PSAN.

  Immunogen:

KLH-conjugated synthetic peptide derived from C-terminal section of PsaN protein in Arabidopsis thaliana AT5G64040.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Applications: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

19 / 12 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity:  

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Vitis vinifera, Gossypium raimondii, Setaria viridis, Zea mays, Sorghum bicolor, Brassica rapa, Brassica napus, Populus trichocarpa, Medicago truncatula, Cucumis sativus, Solanum tuberosum, Nicotiana tabacum, Solanum lycopersicum, Oryza sativa, Setaria viridis, and 80-99% homologues with the sequence in Glycine max, Spinacia oleracea, Hordeum vulgare, Triticum aestivum, Chlamydomonas reinhardtii.
For more species homologues information, please contact tech support at tech@phytoab.com.

1 is thylakoid membrane protein from WT of Arabidopsis thalianacontaining 1 μg of chlorophyll.
2 is thylakoid membrane protein from an Arabidopsis mutant with low accumulation of PSI containing 1 μg of chlorophyll.
Electrophoresis: Tricine-SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.