PsbQ / Anti-PsbQ subunit of oxygen evolving complex of Photosystem II antibody

  Description:

Rabbit polyclonal antibody

  Background:

PsbQ is a 16 kD extrinsic subunit of the oxygen evolving complex of Photosystem II. PsbQ participates in the regulation of oxygen evolution and stabilizes the oxygen evolving complex of PSII. It is required for photoautotrophic growth under conditions of low light.

  Synonyms:

PsbQ, OEC 16 kDa subunit, OEE3, PSBQA,16 kDa subunit of oxygen evolving system of photosystem II, Oxygen-evolving enhancer protein 3-1, chloroplastic

  Immunogen:

KLH-conjugated synthetic peptide derived from N-terminal section of PsbQ protein in Arabidopsis thaliana AT4G21280.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01 M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

24 / 16 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Brassica rapa, Brassica napus, Sorghum bicolor, Hordeum vulgare, Triticum aestivum, Zea mays, Setaria viridis.
For more species homologues information, please contact tech support at tech@phytoab.com.

1 is thylakoid membrane protein from WT of Arabidopsis thalianacontaining 1 μg of chlorophyll.
2 is thylakoid membrane protein from an Arabidopsis mutant with low accumulation of PSII containing 1 μg of chlorophyll.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.