RpoB / Anti-RNA polymerase subunit beta Antibody

In stock

SKU

ATCG00190

Immunogen:	ATCG00190 P50546
Synonyms:	RpoB, RNA POLYMERASE SUBUNIT BETA
Background:	In chloroplasts, transcription of plastid genes is mediated by two types of RNA polymerase: plastid-encoded RNA polymerase (PEP) and nuclear encoded RNA polymerase (NEP). PEP is composed of four core subunits (α, β,β’,β’’) and a promoter recognition subunit (σfactor). RpoB (ATCG00190) is the β subunit of PEP.

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Grouped product items
SKU	Product Description	Size	Type	Purification	Price
PHY1239	RpoB Antibody Data Sheet-PHY1239	150 μg	Rabbit Polyclonal Antibody	Protein A Purified	$318.00
PHY1700	RpoB Antibody Data Sheet-PHY1700	150 μg	Mouse Monoclonal Antibody	Protein A Purified	$388.00
PHY1701	RpoB Antibody Data Sheet-PHY1701	150 μg	Mouse Monoclonal Antibody	Protein A Purified	$388.00
PHY6000	Goat Anti-Rabbit IgG H&L (HRP) Data Sheet-PHY6000	1 ml	Goat Polyclonal Antibody	Protein A Purified	$89.00
PHY6006	Goat Anti-Mouse IgG H&L (HRP) Data Sheet-PHY6006	1 ml	Goat Polyclonal Antibody	Protein A Purified	$89.00

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Details

Product Information

Form:	Lyophilized
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Specificity And Cross Reactions

PHY1239	Arabidopsis thaliana.
PHY1700	Arabidopsis thaliana, Brassica napus, Zea mays, Setaria viridis.
PHY1701	Arabidopsis thaliana, Oryza sativa, Brassica napus, Zea mays, Setaria viridis.
	Black are confirmed Reactivities and Gray are predicted Reactivities.

Reference

2019	mTERF5 Acts as a Transcriptional Pausing Factor to Positively Regulate Transcription of Chloroplast psbEFLJ; Shunhua Ding^1,5, Yi Zhang^1,2,5, Zhi Hu^1,2, Xiahe Huang³, Bohan Zhang^1,2, Qingtao Lu¹, Xiaogang Wen¹, Yingchun Wang^2,3 and Congming Lu^4,; Molecular Plant*; DOI:10.1016/j.molp.2019.05.007.
2019	NCP activates chloroplast transcription by controlling phytochrome-dependent dual nuclear and plastidial switches; Emily J. Yang^1,2,6,8, Chan Yul Yoo^1,8, Jiangxin Liu^3,4,8, He Wang¹, Jun Cao⁵, Fay-Wei Li^2,7, Kathleen M. Pryer², Tai-ping Sun², Detlef Weigel⁵ , Pei Zhou³ & Meng Chen¹; Nature Communications; DOI:10.1038/s41467-019-10517-1.
2019	Phytochrome activates the plastid-encoded RNA polymerase for chloroplast biogenesis via nucleus-to-plastid signaling; Chan Yul Yoo¹, Elise K. Pasoreck¹, He Wang¹, Jun Cao², Gregor M. Blaha³, Detlef Weigel² & Meng Chen¹; Nature Communications; DOI:10.1038/s41467-019-10518-0.
2020	The Arabidopsis mTERF-repeat MDA1 protein plays a dual function in transcription and stabilization of specific chloroplast transcripts within the psbE and ndhH operons; Louis-Valentin Méteignier ¹, Rabea Ghandour ², Karin Meierhoff ³, Aude Zimmerman ¹, Johana Chicher ⁴, Nicolas Baumberger ¹, Abdelmalek Alioua ¹, Jörg Meurer ⁵, Reimo Zoschke ², Kamel Hammani ¹; New Phytologist; DOI: 10.1111/nph.16625.
2020	RHON1 Co-transcriptionally Resolves R-Loops for Arabidopsis Chloroplast Genome Maintenance; Zhuo Yang¹, Mengmeng Li¹ and Qianwen Sun^1,2,; Cell Reports*; DOI:10.1016/j.celrep.2019.12.007.
2020	Accumulation of the RNA polymerase subunit RpoB depends on RNA editing by OsPPR16 and affects chloroplast development during early leaf development in rice; Weifeng Huang¹, Yang Zhang¹, Liqiang Shen^2,3, Qian Fang¹, Qun Liu¹, Chenbo Gong¹, Chen Zhang¹, Yong Zhou⁴, Cui Mao¹, Yongli Zhu¹, Jinghong Zhang¹, Hongping Chen⁵, Yu Zhang², Yongjun Lin¹, Ralph Bock⁶ and Fei Zhou¹; New Phytologist; DOI:10.1111/nph.16769.
2021	RNase H1C collaborates with ssDNA binding proteins WHY1/3 and recombinase RecA1 to fulfill the DNA damage repair in Arabidopsis chloroplasts; Wenjie Wang^1,2 , Kuan Li^1,2 , Zhuo Yang ¹ , Quancan Hou¹ , Wei W. Zhao^1,2 and Qianwen Sun^1,2,; Nucleic Acids Research*; DOI: 10.1093/nar/gkab479.
2021	RCB initiates Arabidopsis thermomorphogenesis by stabilizing the thermoregulator PIF4 in the daytime; Yongjian Qiu^1,2,3✉, Elise K. Pasoreck^1,3, Chan Yul Yoo^1,3, Jiangman He¹, He Wang¹, Abhishesh Bajracharya², Meina Li¹, Haley D. Larsen¹, Stacey Cheung¹ & Meng Chen^1✉; Nature Communications; DOI: 10.1038/s41467-021-22313-x.
2021	A Transcription Factor Regulates Gene Expression in Chloroplasts; Kexing Xin, Ting Pan, Shan Gao and Shunping Yan^; International Journal of Molecular Sciences*; DOI:10.3390/ijms22136769.
2022	Chromatin Immunoprecipitation in Chloroplasts; Wenjie Wang,^1,2,3 Lingling Cheng,^1,3 and Qianwen Sun^1,2,4; Current Protocols; DOI: 10.1002/cpz1.360.
2022	Stromal Protein Chloroplast Development and Biogenesis1 Is Essential for Chloroplast Development and Biogenesis in Arabidopsis thaliana; Weijie Chen^1†, Jingang Huang^1†, Shiwei Chen¹, Lin Zhang¹, Jean-David Rochaix², Lianwei Peng¹ and Qiang Xin^1; Frontiers in Plant Science*; DOI: 10.3389/fpls.2022.815859.
2022	High-resolution map of plastid encoded polymerase binding patterns demonstrates a major role of transcription in chloroplast gene expression; V. Miguel Palomar¹, Sarah Jaksich¹, Sho Fujii^{1, 2}, Jan Kuciński¹ and Andrzej T. Wierzbicki^{1, }; Plant Journal*; DOI: 10.1111/tpj.15882.
2022	Anterograde signaling controls plastid transcription via sigma factors separately from nuclear photosynthesis genes; Youra Hwang ^1,8, Soeun Han^1,5,8, Chan Yul Yoo ^1,6,8, Liu Hong¹, Chenjiang You^1,7, Brandon H. Le¹, Hui Shi², Shangwei Zhong³, Ute Hoecker⁴, Xuemei Chen ¹ & Meng Chen ¹; Nature Communications volume; DOI: 10.1038/s41467-022-35080-0.

2024	Primase promotes the competition between transcription and replication on the same template strand resulting in DNA damage; Weifeng Zhang ^{1 2}, Zhuo Yang ^{1 2}, Wenjie Wang ^{1 2}, Qianwen Sun ^{3 4}; Nature Communications; DOI: 10.1038/s41467-023-44443-0.

2024	pTAC3 and pTAC14 are required for binding of plastid-encoded RNA polymerase to DNA; Joyful Wang¹, V. Miguel Palomar^{1, 2}, Ji-Hee Min¹, and Andrzej T. Wierzbicki¹; biorxiv; DOI: 10.1101/2024.03.01.582956.

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