GLU1 / Anti-Ferredoxin-dependent glutamate synthase 1, chloroplastic/mitochondrial antibody

Ferredoxin-dependent glutamate synthase 1 (also known as Glutamine oxoglutarate aminotransferase) is an enzyme and frequently abbreviated as GOGAT. This enzyme manufactures glutamate from glutamine and α-ketoglutarate, and thus along with glutamine synthetase (abbreviated GS) plays a central role in the regulation of nitrogen assimilation in photosynthetic eukaryotes and prokaryotes. This is of great importance as primary productivity in many marine environments is regulated by the availability of inorganic nitrogen.GOGAT has two forms in plants: ferredoxin-dependent GOGAT (Fd-GOGAT) and NADH-dependent GOGAT (NADH-GOGAT). 95% of GOGAT found in plants is the Fd-GOGAT type. Fd-GOGAT is encoded by two genes, glu1 (AT5G04140) and glu2 (AT2G41220) found on chromosomes 5 and 2 respectively (in Arabidopsis). Fd-GOGAT (both forms) is highly conserved among plants, red algae, and cyanobacteria.

 PhytoAB has PHY0031A antibody, the immunogen is KLH-conjugated synthetic peptide of GLU1 derived from Arabidopsis thaliana AT5G04140.

 Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Brassica rapa, Brassica napus, Spinacia oleracea, Zea mays, Oryza sativa Japonica Group, Hordeum vulgare.

For more species homologues information, please contact tech support at tech@phytoab.com.