Exploring strawberry energy metabolism—with PhytoAB antibodies!
How is SIRT1 activity linked to antioxidant defenses and energy metabolism after harvest?
In Postharvest Biology and Technology, Piechowiak and colleagues investigated strawberry fruit treated with a SIRT1 inhibitor or activator, with and without hydrogen peroxide-induced oxidative stress.
After 24 hours, SIRT1 inhibition was associated with increased oxidative stress and reduced ATP levels. Activation alleviated several biochemical effects of hydrogen peroxide treatment.
The researchers used two PhytoAB antibodies for Western blot analysis of glycolysis-related proteins, with results presented in Figure 4:
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PHY1603A:Hexokinase 1,Reported as HEX1 in the paper; listed as HXK1 in our catalog.
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PHY5395A:PPyruvate kinase 1,Reported as PYK1 in the paper.
Both antibodies were used at a 1:1,000 dilution to examine protein abundance across treatments.
Studying fruit physiology, sugar metabolism, or postharvest stress? Explore PhytoAB antibodies for your protein research.